Generator

Part:BBa_J3103

Designed by: Davidson and Missouri Western   Group: iGEM06_Davidson   (2006-08-01)


pBad-HixC-RBS-TetF-HixC-TT-RE

This part contains the coding region for tetracylcine resistance flanked by Hix C sites. This part is going to be used to test the optimal location of the RBS. If the Hin(BBa_J31000) or Hin LVA (BBa_J31001) genes are placed upstream of this part there is a possibility that the coding region between the Hix C sites is inverted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 509
    Illegal NgoMIV site found at 877
    Illegal NgoMIV site found at 1037
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/hin
Parameters
None